MALONATE DECARBOXYLATION BY PSEUDOMONAS FLUORESCENS
نویسندگان
چکیده
منابع مشابه
Malonate decarboxylation by Pseudomonas fluorescens. II. Magnesium dependency and trapping of active intermediates.
The procedures for culturing the experimental organism, P. jhorescens, and for preparing the crude cell-free extract have been described previously (1). Partial Purification of Crude Enzyme Preparation-Powdered ammonium sulfate was added to a given volume of the crude cell-free extract to obtain 20 per cent saturation. The resulting precipitate was collected by centrifugation for 30 minutes at ...
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It was previously shown that oxygen uptake per mole of substrate, using resting cell suspensions of a strain of Pseudomonas fluorescens, was significantly higher on malonate than on acetate (Wolfe and Rittenberg, 1953). If malonate is anaerobically decarboxylated to carbon dioxide and acetate, one would expect comparable oxygen consumption on the two substrates. The observed difference in uptak...
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Glucose oxidation by Pseudomonas Jluorescens (1) and Pseudomonas aeruginosa (2) has been shown to proceed via gluconate to 2-ketogluconate without the involvement of phosphorylated intermediates. 2-Ketogluconate is oxidized further in growing cultures (3) and resting cell suspensions (4), but attempts to obtain dried cell preparations or cell-free extracts which oxidize 2-ketogluconate have bee...
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MacGee and Doudoroff (2) have gained evidence in support of the postulated scheme by recovering the product of Reaction 1 as a crystalline trisodium salt and establishing its structure as Na3-2-keto-3-deoxy-6-phosphogluconate+2Hz0. Extracts of Pseudomonas Jluorescens catalyze the cleavage of 6-PG to pyruvate and triose phosphate at a rate 10 times faster than the rate of 6-PG dehydrogenase acti...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1954
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)65517-6